目的：观察正常青少年恒河猴麻醉状态下视觉电生理活动的特点，为建立各种疾病的恒河猴动物模型提供更为有效的检查手段和临床参考。 方法：健康36~48月龄恒河猴16只32眼；氯胺酮肌注全麻下应用德国ROLAND CONSULT 公司生产的视觉电生理仪进行f-ERG检测，并分别记录各刺激强度下的检测值；相同方法检测人类正常青少年的f-ERG数据；对以上数据进行分析处理，并将两组数据值进行比较和统计学分析。 结果： f-ERG检测结果显示恒河猴与同龄人类相比较，暗适应状态下不同刺激强度及刺激方式作用下ERG波形的潜伏期均有所缩短，振幅亦有所减小；明适应状态下b波振幅则和人类无显著性差异。 结论：本研究提供了正常恒河猴f-ERG的最佳刺激条件及正常参考值范围。综合分析可知暗适应状态下恒河猴的视网膜电反应弱于正常人类，而明适应状态下电反应活动则与人类无显著性差异。提示我们在对是恒河猴各种眼部疾病动物模型进行以上检测时要更加谨慎的对待恒河猴视网膜电流图正常参考值范围等问题。

AIM: To observe the characteristics of visual electrophysiological activity in normal young rhesus monkeys in anesthesia state, so as to provide more effective checking methods and clinical reference for the establishment of various diseases rhesus animal model. METHODS: Totally 16 healthy rhesus monkeys (32 eyes), 36 to 48-month-old, were selected. Under general anesthesia achieved by intramuscular injection of ketamine, visual electrophysiology instrument(ROLAND CONSULT Co., Ltd) was applied for the detection of flash electroretinogram(f-ERG), and measured values were recorded under different stimulus intensity; The values of f-ERG in normal human adolescents were gained by the same method, then the data were compared and statistically analyzed. RESULTS: The result of f-ERG showed that, compared with the same age of human, the incubation period of ERG in rhesus monkey was shortened and the amplitude was also reduced under different stimulation intensity and stimulation method in dark adaptation state, while in light adaptation the amplitude of b-wave had no significant difference compared with that of human. CONCLUSION: This study provides the optimal stimulation conditions and normal reference range of f-ERG in normal rhesus monkeys. Comprehensive analysis shows that the ERG responses of rhesus monkeys was weaker than normal humans in dark adaptation state, while it has no significant difference in light adaptation state. It reminds us that when we examine the various ophthalmological diseases of rhesus animal model, we should be more careful about the normal reference value scope of ERG.

广州市科技局基金资助项目（No.11C33150706）