β-arrestin1在实验性自身免疫性葡萄膜炎中的表达

Study on the expression of β-arrestin1 in experimental autoimmune uveitis

摘要:

目的:初步探讨β-arrestin1在实验性自身免疫性葡萄膜炎（experimental autoimmune uveitis,EAU）中的表达。 方法：成功建立EAU动物模型后，通过免疫组织化学和RT-PCR的方法检测β-arrestin1在EAU小鼠脾脏细胞中的表达。 结果：免疫组织化学和RT-PCR均提示β-arrestin1在EAU小鼠脾脏细胞中的表达较正常小鼠增高。 结论：β-arrestin1可能在EAU的发生发展中起着重要作用。

Abstract:

AIM：To study the expression of β-arrestin1 in the spleen cells of experimental autoimmune uveitis (EAU). METHODS: The mice were injected into the foot with IRBP R16. On day 3, the spleen was extracted and the single celI suspension was made. Immunohistochemistry and RT-PCR were done on these spleen cells. RESULTS: The expression of β-arrestin1 on the spleen cells of EAU groups was much higher than that in the normal groups. CONCLUSION: β-arrestin1 might play an important role on the EAU.