氯化锂对人眼Tenon 囊成纤维细胞增殖的影响

Inhibitory effects of Lithium chloride on human Tenons capsule fibroblasts

摘要:

目的：观察氯化锂（Lithium chloride）对人眼Tenon囊成纤维细胞（human Tenons capsule fibroblasts, HTFs）体外生长的抑制效应，并探讨其作用机制。 方法：磺基罗丹明（SRB）法和BrdU法检测细胞生长抑制率；采用流式细胞技术（FCM）检测细胞凋亡和细胞周期的变化；Hoechst 33258染色后荧光显微镜观察细胞形态学的改变。 结果：在40~160mmol/L范围内，氯化锂能够抑制HTFs的增殖，且呈剂量与时间依赖性。氯化锂使HTFs阻滞于G₂/M期，且能诱导细胞凋亡，高浓度组尤为明显。 结论：氯化锂能抑制HTFs增殖，机制为诱导细胞凋亡，并将细胞阻滞于G₂/M期。

Abstract:

AIM: To investigate the inhibitory effect of Lithium chloride on human Tenons capsule fibroblasts(HTFs) and explore its mechanism. METHODS: The SRB and BrdU methods were used to observe the activity of HTFs after treated with Lithium chloride in vitro, apoptosis percentage and cell cycle were analyzed by flow cytometry (FCM)．Morphological changes in apoptotic cells were observed by Hoechst 33258 fluorescence staining． RESULTS: The cultured HTFs were significantly inhibited by Lithium chloride in concentration of 40, 80, 160mmol/L compared with the control group and it was dose-dependent and time-dependent．It was demonstrated that Lithium chloride could evoke G₂/M cell cycle arrest and induce apoptosis,especially in the high concentration groups. CONCLUSION: Lithium chloride could restrain growth of HTFs by evoke G₂/M cell cycle arrest and induce apoptosis.