目的：观察轴突生长抑制因子Nogo-A及其受体NgR在视网膜缺血再灌注（retinal ischemia-reperfusion，RIR）急性损伤中的表达，研究两者在RIR损伤中的作用及相关性。 方法：SD大鼠90只随机分为：正常对照组（n=6）；假手术组(n=42)；RIR组（n=42），假手术组及RIR组分为再灌注后0，6，12，24，48，72，168h亚组，每组6只；采用结扎单侧颈总动脉的方法制备大鼠RIR模型，HE染色观察组织形态学改变，免疫组织化学检测Nogo-A和NgR蛋白的表达。 结果：假手术组各时间点Nogo-A及NgR表达与正常组相比无统计学差异（P>0.05），实验组大鼠与假手术组相比：Nogo-A的表达在12h开始升高（P<0.05），48h达到高峰（P<0.01），72h下降（P<0.05），168h达到正常基线水平（P>0.05）；NgR的表达在6h即出现上升，持续至48h达到最高峰（P<0.01），72h下降，168h达到正常基线水平。实验组大鼠Nogo-A与NgR的表达呈正相关（P<0.01）。 结论：Nogo-A和NgR在RIR各时间点均有表达，其表达水平沿时间点呈抛物线形，在再灌注48h时均达到峰值，NgR先于Nogo-A表达，两者协同作用，与RIR损伤后抑制节细胞轴突修复再生有关。

AIM:To observe the expression of Nogo-A and NgR in rat retinal ischemia-reperfusion(RIR) acute injury and to investigate their significance and correlation. METHODS: Ninety adult SD rats were randomly divided into normal group(6 rats), experimental group(RIR group,42 rats) and sham group(42 rats).The experimental group and sham group were subdivided into 0 hour,6,12,24,48,72 and 168 hours subgroups according to different time after RIR injury.Six rats were bring into every subgroup.Ligating the ratssingle common carotid artery was used to establish animal models of rat retinal ischemia. Pathomorphology was detected by hematoxylin and eosin stain and the expression of Nogo-A and NgR was measured by immunohistochemistry. RESULTS: Compared with normal group, the expression of Nogo-A and NgR in sham group had no statistically significant difference (P>0.05). Compared with sham group ,experimental group had the expression of Nogo-A gradually increased in 12 hours after RIR(P<0.05), peaked in 48 hours(P<0.01),decreased in 72 hours(P<0.05) and reached in normal leavel in 168 hours(P>0.05); the expression of NgR increased in 6 hours after RIR(P<0.05), peaked in 48 hours(P<0.01),decreased in 72 hours(P<0.05) and reached in normal leavel in 168 hours(P>0.05). The expression of Nogo-A and NgR in experimental group showed a positive correlation(P<0.01). CONCLUSION: Nogo-A and NgR expressed at each time point after RIR as a parabola.NgR expressed prior to Nogo-A.They were all peaked at 48 hours after RIR.The expression of Nogo-A and NgR closely related to RIR injury, with a collaborative relationship between the two.