目的：通过观察血脂异常ApoE缺失（apolipoprotein E-deficient,ApoE-/-）小鼠视网膜色素上皮（retinal pigment epithelium，RPE）组织及超微结构，探讨健脾祛瘀法对其RPE层VEGF/VEGFR2信号传导通路的影响。 方法：随机将36只2月龄ApoE-/-小鼠分为3组，即：普食组（A组）、高脂组（B组）、治疗组（C组），每组动物12只，喂养5mo后检测其体质量、血脂、血流变，RPE层行光镜、免疫组织化学、透射电镜观察，Western-blot检测VEGF表达。 结果：7月龄ApoE-/-小鼠B组体质量、血脂、血流变指标均高于A组和C组，差异具有统计学意义（P<0.05）。A组RPE厚度明显高于B组和C组，差异具有统计学意义（P<0.05），但B组与C组之间比较差异无统计学意义（P>0.05）。B组RPE层VEGF及VEGFR2染色面积及积分光密度均高于A组和C组，差异具有统计学意义（P<0.05）。B组视网膜VEGF蛋白的相对表达量明显高于A组和C组，差异具有统计学意义（P<0.05）。 结论:健脾祛瘀法对改善血脂异常ApoE-/-小鼠的血脂、血流变等生化指标有积极作用，并且提示对该AMD动物模型中RPE层VEGF/VEGFR2信号传导通路有一定调控作用。

AIM: To research tissue and ultrastructure in retinal pigment epithelium（RPE） of apolipoprotein E-deficient(ApoE-/-) mice effected by dyslipidemia in using jianpiquyu method,and to explore the effects on the VEGF/VEGFR2 pathway. METHODS: Thirty-six 2-month-old ApoE-/-mice were randomly divided into three groups:the group of normal chow(group A);the group of high fat chow(group B)，the group of treatment(group C),twelve mice in each group.Animals were killed after five months feeding and animal bodymass,blood lipid and hemorheology levels were detected．RPE was observed by light microscopy,transmission electron microscopy and immunohistochemistry． And the VEGF were analysed by Western blot. RESULTS: The animal bodymass,lipid and hemorheology levels in group B were significantly higher than those of group A and group C(P＜0.05).The thickness of RPE in group A was statistically higher than that of group B and group C(P＜0.05)，but there was not statistically signigicant difference between group B and group C（P>0.05）.VEGF and VEGFR2 dyeing area and intergral optical density in group B were higher than those of group A and group C(P＜0.05).Protein expression in retina in group B was higher than that of group A and group C(P＜0.05). CONCLUSION: It plays a positive role in ameliorating biochemical indicators such as lipid and hemorheology levels in dyslipidemia ApoE-/-mice by using jianpiquyu method.It also plays a regulatory function in the VEGF/VEGFR2 pathway in this age-related macular degeneration (AMD) animal model.

国家自然科学基金资助项目（No.30973774）