目的：研究Pax2及Sox2和Prox1在小鼠眼胚胎发育过程中的时空表达模式及相互关系，探讨其可能的作用。 方法：取不同发育阶段的胚胎小鼠眼的中轴冰冻切片，采用免疫荧光共标记技术，观察Pax2和Sox2及Prox1在眼内表达分布规律及相互关系。 结果:在胚胎期9.5d（E9.5），Sox2表达在视泡和晶状体板，而Pax2表达在视泡前体细胞中，而Prox1仅在晶状体板中弱表达。 E11.5时，Sox2在晶状体泡和原始视网膜均呈阳性，而Pax2仅在视柄和视柄邻近的腹侧视网膜及色素上皮层内表达，Prox1主要集中在晶状体泡后壁细胞内。到E12.5后，Sox2仍广泛分布在视网膜和晶状体中，但晶状体的表达强度明显弱于视网膜区域的表达；Pax2仅表达在视柄及分化形成的视神经中；而Prox1逐渐局限在原始晶状体纤维细胞及晶状体上皮细胞中。 结论:Pax2和Sox2及Prox1参与小鼠眼的发育，它们之间互相联系但有明显不同的时空分布特点，提示在眼的发育中可能具有不同的功能。
AIM: To investigate the relationships of the temporal and spatial expression profiles of Pax2, Sox2 and Prox1 during eye development in the embryonic mouse, and to gain further insight into their possible functions. METHODS: Time-mated embryonic C57/6 mice were killed at different developmental stages. The eye tissues were embedded in OCT and sectioned at 10μm. The sections were then mutually colabeled with Pax2, Sox2 or Prox1 and observed by fluorescence microscope. RESULTS: On E9.5, Sox2 transcripts were initially distributed throughout optic vesicle and lens placode. However, Pax2 was only expressed in the entire optic vesicle and Prox1 in the lens placode. At E11.5, Sox2 was still distributed throughout presumptive retina and lens vesicle. But Pax2 was more restricted to the proximal portions of the optic cup and stalk, while Prox1 was in the posterior portion of the lens vesicle. After E12.5, Sox2 was still distributed throughout retina and lens vesicle, but the positive expression intensity in lens vesicle was much lower in the retina. Meanwhile, Pax2 was only restricted in the optic stalk and Prox1 was gradually limited in the presumptive lens epithelium and the primary lens fibers. CONCLUSION: Expression patterns of Pax2，Sox2 and Prox1 were different, and they were overlapped during eye development in the embryonic mouse. The different expression patterns in eye which suggest that they play different roles in the developing eye may help to shape each respective structure.
陈裕庆,刘少峰. Pax2和Sox2及Prox1在胚胎小鼠眼发育中的表达模式.国际眼科杂志 2012;12(5):819-822复制