目的：分离、培养与检测年龄相关性白内障房水中的纳米细菌(nanobacteria,NB),为进一步探讨NB致白内障的机制奠定基础。 方法：白内障超声乳化联合人工晶状体植入手术术前收集30份房水标本,通过脱矿、过滤、离心处理,用细胞培养的方法进行NB培养,观察其生长情况。采用免疫组织化学物形。 结果：培养6wk后,对培养标本进行观察,发现12瓶(40%)培养液中存在絮状漂浮成团的NB,并且其中8瓶培养液瓶底亦可见贴壁生长的成团的或散在分布的颗粒沉淀状NB；对照组一看见1例絮状漂浮，并可见管底颗粒沉淀；对照组二未见絮状漂浮和颗粒沉淀。透射电镜可见NB为针状物的聚集体，大小不一。 结论：从年龄相关性白内障患者房水中分离培养鉴定出NB,表明其感染与白内障发病可能有关,需进一步研究其致病机制。

AIM:To isolate, culture and identify the nanobacteria (NB) from aqueous humor of patients with age-related cataract in our department. METHODS:Sample nanobacteria in aqueous humor of 30 patients with age-related cataract were collected for NB culture in this study. Immunohistochemistry, transmission electron microscopy (TEM) were used to investigate the appearance and components of cultured NB. RESULTS:Twelve aqueous samples out of 30 (40%) showed growth of NB, 1 of 5 showed growth of NB in the saline control group, but none growth in the control group. TEM showed needle-like nanobacteria aggregates of different sizes. CONCLUSION:For the first time we isolated, cultured and identified nanobacteria in aqueous of patients with age-related cataract, indicating that nanobacteria infection is related with age-related cataract.

中国广州市番禺区科技攻关计划资助项目（No.2009-Z-123-1）