目的:研究不同培养基对兔晶状体上皮细胞(LEC)培养的影响。方法:使用组织块贴壁法原代培养兔LEC,倒置显微镜观察不同培养基(DMEM低糖、高糖、F12)下兔LEC形态、生长速度的情况。结果:DMEM低糖和高糖培养基使培养2wk后的细胞开始出现分化,并停止生长,晶状体上皮细胞明显成纤维细胞化。DMEM/F12培养基使细胞生长良好,传至第5代时细胞开始发生转化变为成纤维细胞。结论:DMEM低糖和高糖培养基造成兔LEC增殖抑制,DMEM/F12培养基适合兔LEC的生长。

AIM:To study the effect of different media on the original rabbit lens epithelial cells·METHODS:Using the tissue block adhering wall method to culture the rabbit lens epithelial cells(RLEC) original generation in vitro,the situation of morphology,proliferation and differentiation of the original RLEC in the media containing different ingredients(DMEM low glucose,DMEM high glucose and DMEM/F12 medium) were learned·RESULTS:Cells cultured for 2 weeks on DMEM low and high glucose media began to differentiate and stop growing,the lens epithelial cells had significant fibrosis.DMEM/F12 medium made the cells grow well.When the cells passed on to the 5th generation,they began to transform into fibroblasts·CONCLUSION: The results indicates that the DMEM low glucose and DMEM high glucose media could inhibit the proliferation of the original RLEC to some extent,and DMED/F12 medium is suitable for the proliferation of original RLEC.

R776.1

中国国家自然科学基金资助项目(No.81001577,No.81072961)