观察茶多酚(tea polyphenols,TP)对紫外线诱导的大鼠晶状体上皮细胞中Caveolin-1表达的影响。方法:采用离体晶状体培养技术,通过紫外线照射氧化损伤法,建立实验性白内障晶状体模型,设置空白对照组、紫外线照射组和实验组(即紫外线照射+TP组),实验组按TP的浓度分为4个亚组,即0.02,0.2,2和20mg/L,分别于6,12,24和48h取样,运用免疫组织化学法染色计数晶状体上皮细胞中Caveolin-1阳性细胞,并运用Western-blot免疫印迹分析晶状体上皮细胞中Caveolin-1表达量的变化。结果:不同时段各组晶状体上皮细胞Caveolin-1阳性表达率和表达量的比较,各实验组与紫外线对照组相比,差异均有统计学意义(P<0.05),不同时段实验组晶状体上皮细胞阳性表达明显高于紫外线照射组,实验组的晶状体混浊程度明显低于紫外线照射组。0.02mg/L TP实验组与空白对照组相比,差异无统计学意义(P>0.05);其余实验组与空白对照组相比,差异均有统计学意义(P<0.05)。结论:TP可以抑制紫外线诱导的晶状体上皮细胞Caveolin-1表达的减少,其中0.02mg/L TP对Caveolin-1的作用最强。

AIM:To observe the influence of tea polyphenols(TP)on the Caveolin-1(CAV-1) expression in ultraviolet(UV)-induced lens epithelial cells of rats.METHODS:The experimental cataract model was established,and the untreated group,ultraviolet radiation group and experimental group(ultraviolet radiation + TP group) were set.The experimental group by tea polyphenols concentration was divided into four subgroups,namely 0.02,0.2,2 and 20mg/L group respectively,with sampling 6,12,24,48 hours after culture respectively.The expressions of CAV-1 were detected by the S-P immunohistochemical method and Western blot method in lens epithelial cells.RESULTS:The differences of Caveolin-1 expression for the experimental group and control group compared to the UV group were statistically significant(P<0.05).At different times,the lens epithelial cells positive expression for the experimental group was significantly higher than the UV group,the degree of lens opacity for the experimental group was significantly lower than the UV group.When TP was 0.02mg/L,the difference of Caveolin-1 expression between the experimental group and the control group was not statistically significant(P>0.05);the difference between the rest of the experimental group and the control group was statistically significant(P<0.05).CONCLUSION:Tea polyphenols can inhibit the expression of Caveolin-1 decreased in UV-induced lens epithelial cells.When tea polyphenols concentration is 0.02mg/L,the effect is the best.

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