目的:探讨飞秒激光在离体猪眼角膜行板层切削的切削深度、平滑度及可行性。方法:新鲜离体猪眼及甘油保存复水猪眼各10只,利用飞秒激光进行板层切削。激光切削前后对角膜进行测厚,计算角膜瓣厚度。同时选择徒手制作同样直径角膜瓣,扫描电镜观察两种方法制瓣的角膜基质面的形态区别。结果:新鲜离体猪眼角膜瓣厚度为394.10±12.04μm,甘油保存复水后猪眼角膜瓣厚度为366.30±15.28μm。飞秒激光切削的角膜表面较徒手剥离的角膜表面光滑。结论:飞秒激光在离体猪眼表面切削深度与设定切削深度存在差异,其切削表面光滑,在角膜移植中具有广泛应用前景。

AIM:To evaluate the cutting depth,smoothness and feasibility of corneal laminal cutting with femtosecond laser on porcin eye.METHODS:Corneal laminal cutting with femtosecond laser was performed on 10 fresh in vitro porcin eyes and 10 rehydrated glycerin preserved procin eyes.Thickness of cornea and corneal flap were measured and calculated before and after laser cutting.Meanwhile,corneal flap with same diameter was made manually.Two types of cutting surfaces were observed with scanning electron microscope.RESULTS:Thickness of flap in fresh cornea and rehydrated cornea was 394.10±12.04μm and 366.30±15.28μm respectively.Cutting surfaces with laser was more smooth than that of manually made.CONCLUSION:There is difference between actual and predetermined cutting depth with femtosecond laser in vitro porcin cornea.Cutting surfaces with laser was more smooth.There should be better prospect in keratoplasty.

R779.63