目的:观察丹参酮ⅡA对体外培养人翼状胬肉成纤维细胞(HPF)增殖和凋亡的影响,寻找辅助治疗和预防翼状胬肉复发的新途径。方法:用0～320mg/L丹参酮ⅡA作用体外培养的HPF,24～96h后MTT法检测药物对HPF的影响,免疫组织化学染色增生细胞核抗原(PCNA)检测细胞生长活性。结果:80mg/L丹参酮ⅡA作用48h后能显著抑制HPF的增殖(P<0.05),呈剂量和时间依赖性。丹参酮ⅡA在80～320mg/L范围内能浓度依赖性地抑制细胞表达PCNA(P<0.05)。结论:丹参酮ⅡA可显著抑制HPF的增殖。

AIM:To investigate the effect of tanshinone ⅡA on proliferation and apoptosis of human pterygium fibroblasts (HPF) in culture and search for a new method to prevent the recurrence after pterygium surgery.METHODS:HPF was incubated with 0-320mg/L tanshinone ⅡA for 24-96 hours.The MTT method was used to assay the biologic activities of the tanshinone ⅡA in different time and different doses. The expression of proliferating cell nuclear antigen (PCNA) in each group was detected by immunohistochemical staining. The cell cycle distribution was measured by flow cytometic analysis. RESULTS: Administration of 80mg/L tanshinone ⅡA for 48 hours could significantly inhibit HPF proliferation in a dose-and time-dependent manner (P<0.05).Tanshinone ⅡA (80-320mg/L) could inhibit the expression of PCNA in HPF in a dose-dependent manner (P<0.05). CONCLUSION: Tanshinone ⅡA can significantly inhibit HPF proliferation and induce its apoptosis.

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