目的:探讨银杏叶提取物(extractof Ginkgo bilobaleaves,EGb)对大鼠持续性高眼压损伤下的视网膜神经节细胞(retinalganglioncells,RGC)活性的保护作用。方法:健康SD大鼠20只,采用烧烙法,烙闭大鼠左眼2条浅层巩膜静脉,制作大鼠持续性高眼压模型,从中选出眼压稳定在实验要求(眼压>26mmHg)的大鼠12只随机分为A组(对照组)和B组(EGb治疗组)。治疗组予EGb每日150mg/kg灌胃治疗,对照组为空白对照,不做任何处理。1mo后处死大鼠摘取眼球,制作眼球标本,常规石蜡切片,AgNOR染色观察RGC细胞核内的银染颗粒。采用计算机图象分析系统对RGC细胞核内的银染颗粒进行定量分析。结果:A组大鼠模型眼RGC细胞核内银染颗粒为1.33±0.07个/细胞,B组为1.83±0.09个/细胞,B组模型眼较A组模型眼RGC细胞核内银染颗粒多,其差异有显著性意义(P<0.05)。两组模型眼较正常眼的RGC细胞核内银染颗粒明显减少,其差异有显著性意义(P<0.05)。结论:大鼠持续性高眼压可导致RGC细胞核内银染颗粒减少,而EGb对RGC活性有部分保护作用。

AIM:To investigate the protective effect of extract of ginkgo biloba leaves(EGb)on the activity of retinal ganglion cells(RGC)in the continuous high intraocular pressure(IOP) SD rats.METHODS:Totally 20 healthy SD rats were established continuous high IOP models by cauterizing two episcleral vessels in the left eye. Then 12 rats satisfying experimental level(IOP>26mmHg) were selected and divided randomly into two groups, group A(control group) and group B(EGb treated group), 6 rats in each group. EGb was irrigated into the stomachs of rats in the treated group at the dose of 150mg/kg per day. The rat eyes were excised and made into eyeball samples after one month.The paraffin-embedded slides were stained with silver staining for nucleolar organizer region(AgNOR) to observe and count stained grains in RGC nucleus. RESULTS: Stained grains of RGC nucleus in model eye of group A was 1.33±0.07/cell,and group B was 1.83±0.09/cell, There was significant difference between group A and group B(P<0.05). Stained grains were more in group B than group A. Stained grains in RGC nucleus of the high IOP with EGb treated subgroup were more than those of the simple high IOP eyes. Stained grains were obviously decreased in the high IOP eye according to the normal IOP eye. Statistical significance was found between the high IOP eye and the normal IOP eye in the two groups (P<0.05) .CONCLUSION: Continuous high IOP in rats by cauterizing the episcleral vessels can result in the decrease of stained grains in RGC nucleus. EGb may have partial protective effect in RGC activity of high IOP rats.

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