目的:研究钙调素拮抗剂小檗胺(berbamine,BER)对兔Tenon囊成纤维细胞和兔小梁切除术后滤过道内成纤维细胞增殖的抑制作用。方法:体外培养兔Tenon囊成纤维细胞,经不同浓度BER处理不同时间后,细胞计数Kit8(CCK8)法检测BER对兔Tenon囊成纤维细胞增殖的抑制作用,流式细胞仪检测其凋亡率及细胞周期的变化。HE染色检测BER对兔小梁切除术后滤过道内成纤维细胞增殖的抑制作用。结果:兔Tenon囊成纤维细胞经不同浓度BER处理不同时间后细胞增殖受到抑制,并呈时间剂量依赖性。流式细胞仪检测发现BER处理后兔Tenon囊成纤维细胞呈现G1期阻滞,细胞凋亡率明显增加,20mg/LBER处理9h,细胞凋亡率从0.64%增加到31.86%。HE染色显示BER显著抑制兔小梁切除术后滤过道内成纤维细胞的增殖。结论:小檗胺在体内外均能抑制成纤维细胞的增殖,其可能是通过诱导细胞凋亡方式抑制兔Tenon囊和滤过道内成纤维细胞的增殖。

AIM:To investigate the inhibitory effects of berbamine on fibroblasts in Tenon’s capsule and filtering channel of rabbits.METHODS: Rabbit Tenon’s capsule fibroblasts were treated with berbamine at different concentrations (0-100mg/L) for different hours. CCK8 assay was used to determine proliferation inhibition of rabbit Tenon’s capsule fibroblasts. Flow cytometry was performed to examine cell cycle profile and cell apoptosis rate in response to berbamine treatment. Conjunctive and sclera of filtering area of the rabbits suffered trabeculectomy were examined by pathologic histology examination.RESULTS: Berbamine inhibited the proliferation of rabbit Tenon’s capsule fibroblasts in a dose-and time-dependent manner. Flow cytometric analysis revealed that Tenon’s capsule fibroblasts treated by berbamine were arrested at G1 phase and apoptotic cells increased from 0.64% to 31.86% after treatment with berbamine at 20mg/L for 9 hours (P<0.01). HE stain showed fibroblasts in filtering channel of rabbit were inhibited significantly by berbamine.CONCLUSION: Berbamine could inhibit the growth of fibroblasts in vitro and vivo in rabbit Tenon’s capsule and filtering channel. The induction of cell apoptosis might be one of the mechanisms for suppressing the growth of fibroblasts.

R779.6