樟柳碱联合地塞米松治疗对大鼠视神经挫伤后F-VEP和ERG的影响

Effect of anisodine combined with dexamethasone treatment on F-VEP and ERG of rats after the optic nerve contusion

摘要:

目的:观察SD大鼠视神经挫伤后樟柳碱(anisodine,AT3)联合地塞米松(dexamethasone,Dex)治疗对闪光视觉诱发电位(flash visual evoked potential,F-VEP)和视网膜电图(electroretinogram,ERG)的影响。方法:成年雌性SD大鼠30只,均以左眼为实验眼,采用随机数字法分为A,B,C共3组,利用压力恒定的无创反向镊夹持大鼠视神经6s建立视神经挫伤模型,A组建立模型前1d开始每日给予尾静脉注射0.25mL(0.5mg)Dex,同时左眼球后注射0.25mLAT-3;B组尾静脉注射同A组,但球后注射生理盐水;C组静脉及球后均给予生理盐水。记录并比较3组大鼠建模前及建模后0.5,1,7,14d的F-VEP的P1波和ERG的b波振幅和峰潜时变化。结果:C组视神经损伤0.5d后,F-VEP振幅值降低与正常相比有显著性差异,峰潜时在1d后与正常相比有统计学意义,并且在观察时间内随时间其峰潜时和波幅呈进行性下降。B组在7d后F-VEP振幅值明显高于与对照组(P=0.031),峰潜时短于对照组(P=0.012),C组在7d后F-VEP振幅值明显高于对照组(P=0.042),峰潜时短于对照组(P=0.019)。结论:AT3联合Dex对大鼠视神经挫伤后神经传导功能的恢复较单纯应用Dex明显。

Abstract:

AIM:To observe the impact of anisodine (AT3) combined with dexamethasone (Dex) treatment on the flash visual evoked potentials ( F-VEP) and electroretinogram (ERG) of SD rats after optic nerve contusionMETHODS: Thirty adult female SD rats,taken their left eyes as experimental eyes, were divided into A, B, C groups using random numbers, and gripped optic nerve 6 seconds with noninvasive pressure-constant reverse tweezers to establish optic nerve contusion models.Group A was given caudal vein injection of 0.25mL (0.5mg) Dex one day before setting up the model, as well as retrobulbar injection of 0.25mL AT3 in the left eye; Caudal veins of group B was injected the same amount of normal saline by retrobulbar injection; Group C was given both intravenous and retrobulbar injection of normal saline. P1 wave of the F-VEP and b-wave amplitude and peak latency changes of ERG in 3 groups were recorded and compared before and 0.5,1 day,7,14 days after modeling.RESULTS: In group C , 0.5 day after optic nerve injury, F-VEP amplitude decreased significantly when compared with the normal . Peak latency 1 day after modeling had statistical significance when compared with the normal, and peak latency and amplitude decreased progressively during the observation time. In group B 7 days after modeling, the F-VEP amplitude was significantly higher than that in the control group (P=0.031), peak latency shorter than the control group (P=0.012). In group C 7 days after modeling, the F-VEP amplitude was significantly higher than that in the control group (P=0.042), peak latency shorter than the control group (P=0.019).CONCLUSION:Curative effect of AT3 combined with Dex on the recovery of nerve conduction after optic nerve contusion in rats is significantly higher than that of alone applications of Dex.