白藜芦醇对兔急性高眼压视网膜节细胞NF-κB表达和IL-6浓度的影响

Effect of resveratrol on expression of NF-κB and IL-6 concentration in acute ocular hypertension rabbits model

摘要:

目的:观察白藜芦醇(resveratrol,Res)对兔急性高眼压条件下视网膜节细胞(retinal ganglial cells,RGC)核因子-κB(nuclear factor-kappe B,NF-κB)表达及血清中白细胞介素-6(interleukin-6,IL-6)浓度的影响。方法:将新西兰大白兔随机分成处理组、对照组和正常组。处理组于急性高眼压模型建立前7d,每天给予Res30mg/kg(3mL)ip,第8d经前房灌注升高眼压达90mmHg(1kPa=7.5mmHg),持续90min,24h后取材;对照组用等剂量的生理盐水代替Res给予ip,余同处理组;正常组不做上述处理直接取材。观察兔视网膜组织病理学结果,免疫组织化学检测RGC中NF-κB表达及血清中IL-6浓度的变化。结果:与正常组相比,处理组和对照组表达在RGC的NF-κB明显增加,外周血清中IL-6浓度明显增高(P<0.05);与对照组相比,处理组NF-κB的表达明显减少,外周血清中IL-6浓度明显降低(P<0.05)。结论:Res通过降低兔急性高眼压RGC中NF-κB的表达和血清中IL-6浓度,减轻RGC的损伤,对视网膜具有一定的保护作用。

Abstract:

AIM:To observe the effect of resveratrol on expression of nuclear factor-kappe B (NF-κB) and IL-6 concentration in acute ocular hypertension rabbits model.METHODS:The New Zealand rabbits were randomly divided into treated group, control group and normal group. Before setting up the model of acute high intraocular pressure,the resveratrol 30mg/kg(3mL) was injected into abdominal cavity of treated group’s rabbits for 7 days, an acute ocular hypertension model of 90 mmHg (1kPa=7.5mmHg) was induced after 90 minutes’ anterior chamber infusion at the eighth day . Specimen was obtained at 24 hours after the ocular hypertension. In control group normal saline was used instead of resveratrol; For the normal group, specimen was obtained without the above treatment. The specimen was removed for microscopic examination, and the expression of NF-κB was studied by immunohistochemical examination, and blood serum was collected to detect IL-6 concentration.RESULTS:Compared with the normal group, the expression of NF-κB in retinal ganglial cells(RGC) and IL-6 concentration in treated group and control group were significantly up-regulated; Compared with the control group, the expression of NF-κB in RGC and IL-6 concentration in treated group were significantly decreased.CONCLUSION:Through decreasing the expression of NF-κB and IL-6 concentration, resveratrol can reduce the RGC damages. It may play a protective role on the retina in glaucoma pathology.