目的:通过研究葡萄膜炎患者的外周血单个核细胞分泌的多个细胞因子水平,探讨葡萄膜炎可能的发病机制。方法:取30例葡萄膜炎患者及20例健康对照外周血,分离出单个核细胞后短期培养,通过酶联免疫反应(ELISA)检测上清IFN-γ,TNF-α和IL-10的水平。结果:葡萄膜炎患者外周血单个核细胞分泌的IFN-γ和TNF-α含量较正常对照显著增高(P<0.05),而且与葡萄膜炎患者病情严重程度密切相关;IL-10在葡萄膜炎患者外周血单个核细胞培养上清中表达水平较健康对照略高,无统计学差异(P>0.05)。结论:本实验证实了葡萄膜炎患者外周血单个核细胞分泌的IFN-γ,TNF-α水平较高,与病情相关,提示其在葡萄膜炎发病中的重要作用。

·AIM:To study the possible mechanism of uveitis by evaluating the expression of cytokines produced by peripheral blood mononuclear cells.·METHODS:Peripheral blood was drawn from 30 patients with uveitis and 20 normal people.After separation and short term culture of the mononuclear cells,the amount of IFN-γ,TNF-α and IL-10 in the supernatant were evaluated respectively with ELlSA.·RESULTS:The amount of IFN-γ and TNF-α produced by peripheral blood mononuclear cells from the patients were much higher than that in the normal people(P<0.05),and they both correlated well with disease activity of uveitis.IL-10 expression in that of uveitis was little higher than normal control with no statistical significance.·CONCLUSION:The elevated expression of IFN-γ and TNF-α by peripheral blood mononuclear cells may play important role in the pathogenesis of uveitis.·

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