目的:探讨IL-10修饰的供体树突状细胞(dendritic cells,DC)在大鼠角膜移植中的作用。方法:建立Wistar-SD大鼠穿透性角膜移植动物模型,Wistar大鼠为供体,SD大鼠为受体。受体大鼠42只随机分为3组,每组14只。A组为对照组,受体不经任何预处理,即行角膜移植术;B组角膜移植前3d,每只受体鼠经尾静脉注射供体的未成熟DC,细胞数为2×107个;C组角膜移植前3d,每只受体鼠经尾静脉注射经IL-10修饰的供体未成熟DC,细胞数为2×107个。结果:以混浊、水肿和新生血管3项指标作为临床评估标准,观察各组角膜植片的存活时间。角膜植片的存活时间3组分别为:10.1±1.7,17.8±1.8,26.4±1.6d。各干预组与对照组比较差异均有显著性(P<0.01)。B,C组间比较差异有显著性(P<0.01)。结论:摄取供体抗原的不成熟DC能显著延长角膜植片的存活时间;IL-10修饰的DC可进一步延长角膜植片的存活时间。

AIM:To study the effect of donor IL-10 modified dendritic cells(DC)for providing theoretical and practical basis for anti-rejection of corneal transplantation.METHODS:Wistar rats were used as donors,and SD rats were used as recipients.The recipient was injected by tail vein with donor IL-10-treated DC or with untreated DC.Keratoplasty was performed after three days and the survival time of corneal allografts was observed.Animals were randomly assigned to the following three groups:(A)control,(B)DC,(C)IL-10-DC.RESULTS:The mean survival time in the control group was merely 10.1±1.7 days,but in the pretreated groups was 17.8±1.8 days and 26.4±1.6 days respectively which was statistically prolonged compared with the control groups(P<0.01),and comparison within the two pretreated groups was significantly different(P<0.01).CONCLUSION:It is indicated that pretreatment with donor immature DC could markedly prolong the survival time of corneal allografts.And IL-10-modified DC can further extend the rat corneal allografts.

R779.65

中国辽宁省教育厅重点实验室资助项目(No.IS2010103)