目的:研究细胞因子VEGF,PDGF,Angiogenin和ET-1在GK大鼠糖尿病视网膜病变(diabetic retinopathy,DR)早期静脉血中的表达情况。方法:选用GK大鼠作为DR模型动物,正常Wistar大鼠作为对照。依据视网膜铺片和HE染色切片以明确眼底病变阶段,将两组大鼠分别分为2,4,6月龄3组。ELISA法检测各组大鼠静脉血中VEGF,PDGF-BB,Angiogenin和ET-1的浓度。结果:GK大鼠静脉血中的VEGF,Angiogenin和ET-1含量均高于正常Wistar大鼠(P<0.05);GK大鼠静脉血中ET-1浓度随着月龄的增加而升高(P<0.05);静脉血中PDGF-BB浓度的变异度较大,组间比较无统计学意义。结论:静脉血中VEGF,ET-1和Angiogenin含量的变化可作为早期DR病情监测的指标。

AIM:To investigate the expression of cytokines:vascular endothelial growth factor(VEGF),platelet-derived growth factor(PDGF),angiogenin,endothelin-1(ET-1)in venous blood of diabetic retinopathy rat models in early stage.METHODS:GK rats were chosen as the DR animal models and the normal Wistar rats as the control group.The rats were divided into 3 groups:aged 2,4,6 months old.Concentration of VEGF,PDGF-BB,angiogenin,ET-1 in venous blood were detected by ELISA.RESULTS:The expression of VEGF,angiogenin,ET-1 in venous blood of GK rats was significantly higher than that of normal Wistar rats(P<0.05).The concentration of ET-1 in venous blood of GK rats became higher as animal grew(P<0.05).There were no statistical differences in PDGF-BB of venous blood between the groups of rats.CONCLUSION:The expression of VEGF,ET-1,angiogenin in venous blood may be used to follow up the early stage of DR.Because of the obvious fluctuation of PDGF-BB in venous blood,we are not sure of its value.

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中国上海市科学技术委员会自然科学基金资助项目（No.10ZR1418500）;中国上海市宝山区科学技术委员会自然科学基金资助项目（No.08-E-09）