目的:探讨土贝母皂甙(tubeimoside,Tu)对兔单纯疱疹病毒性角膜炎(herpes simplex keratitis,HSK)作用的效果。方法:选取24只兔建立HSK模型,随机分4组,即生理盐水、阿昔洛韦、Tu和Tu联合应用重组牛碱性成纤维细胞生长因子(recombinant bovine basic fibroblast growth factor,bFGF)。裂隙灯显微镜、扫描电镜和透射电镜观察眼部病变的动态变化。8只兔左眼滴0.4g/L Tu,右眼滴生理盐水。结果:Tu、阿昔洛韦、Tu+bFGF与生理盐水4组间比较,角膜上皮病变分值(corneal epithelial involvement scores,CEIS)均有显著性差异(q=7.75,20.50,22.17,P<0.01)。阿昔洛韦组和Tu组、Tu+bFGF组相比,CEIS差异有显著性(t=2.1,1.9,P<0.05)。Tu组与Tu+bFGF相比,CEIS有显著性差异(t=2.17,P<0.05)。结论:采用0.4g/L Tu治疗实验性HSK有一定的疗效,联合应用bFGF可以增强Tu治疗HSK的疗效。0.4g/L Tu滴眼有轻度刺激性。

AIM:To evaluate the efficacy of tubeimoside(Tu)on herpes simplex keratitis(HSK)of rabbits.METHODS:HSK models were established in 24 rabbits,which were randomly divided into 4 groups:normal saline(NS),acyclovir(ACV),Tu and Tu combined with recombinant bovine basic fibroblast growth factor(bFGF).All eyes were examined and corneal samples’ lesions dynamic changes were selectively observed by slit-lamp microscope,scanning and transmission electron microscope.RESULTS:Among 4 groups of NS,ACV,Tu and Tu +bFGF,corneal epithelial involvement scores(CEIS)showed significant difference(P<0.01);compared ACV with Tu or Tu +bFGF respectively,CEIS had significant difference(P<0.05),as well as that between Tu and Tu +bFGF(P<0.05).CONCLUSION:There is curative effect on HSK with Tu(0.4g/L),and the therapeutic effect can be enhanced when combined with bFGF except for slight stimulatory.

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