复方樟柳碱对大鼠视网膜缺血再灌注损伤的保护作用

Protection effect of compound anisodine in rats with retinal ischemia-reperfusion injury

摘要:

目的:探讨复方樟柳碱对大鼠视网膜缺血再灌注损伤的治疗作用。方法:将成年雌性Wistar大鼠随机分成正常对照组,造模组和复方樟柳碱组,每组10只。采用前房灌注升高眼压制作急性视网膜缺血模型。观察1,3,7d视网膜电图a波b波的恢复情况。每组随机取2只大鼠造模眼球光镜、电镜下观察视网膜形态结构的变化,以及神经节细胞线粒体的变化。结果:复方樟柳碱组较造模组神经节细胞线粒体肿胀程度轻,b波明显恢复,同造模组与正常组间b波的恢复比较差异有显著统计学意义(P<0.01)。结论:复方樟柳碱可减轻大鼠视网膜缺血再灌注损伤,有神经细胞保护作用。

Abstract:

AIM:To investigate the therapeutic effect of compound anisodine in rats with retinal ischemia-reperfusion injury.·METHODS:Totally 30 adult female rats were divided into three groups at random. Group 1(n=10):normal control;group 2(n=10):high intraocular pressure (IOP)induced ocular ischemia model; group 3(n=10):compound anisodine treatment. At the 1 day,3,7 days the recovery condition of a-wave and b-wave by electroretinogram were observed in each group respectively. The morphology of retinal were observed by light microscope(n=2) in each group randomly. Retinal morphology and ganglion cells mitochondrion were evaluated by electron microscope.·RESULTS:In our research,the recovery of b-wave in group 3 were better than that in group 1 and 2,and showed significant difference(P<0.01).In addition,group 3 ganglion cells mitochondrion swelling were lighter than that in group 2.·CONCLUSION:Compound anisodine can ameliorate the retinal ischemia-reperfusion injury in rats.