目的:观察色素上皮细胞衍生因子(pigment epithelium-derived factor,PEDF)在人类白内障晶状体上皮细胞中的表达,以探讨PEDF在白内障发病机制中的作用。方法:白内障超声乳化手术中,实施前囊膜环形撕囊时摘取晶状体前囊连同晶状体上皮细胞,采用免疫组化的方法检测PEDF和VEGF在晶状体上皮细胞中的表达。结果:老年性白内障晶状体上皮细胞虽然PEDF存在高强度表达,与VEGF相比,这种表达还是较弱。结论:老年性白内障晶状体上皮细胞存在PEDF和VEGF的表达,而且二者之间存在着一定的相互作用,由此推断PEDF和VEGF在老年性白内障的发病机制中处于重要的作用。

AIM:To observe the expression of pigment epithelium-derived factor (PEDF) in human cataract lens epithelial cells,and to explore its role in the pathogenesis of cataract.·METHODS:During the cataract circular capsulorhexis of phacoemulsification surgery,the anterior lens capsule and lens epithelial cells were obtained and fixed. Immunohistochemical method was used to determine the expression of of PEDF and VEGF in lens epithelial cells.·RESULTS:There were expression of PEDF and VEGF in senile cataract crystal epithelial cells,while the high expression of PEDF was weaker than that in the VEGF.·CONCLUSION:PEDF and VEGF are expressed in senile cataract lens epithelial cells and both exist certain interactions. It may plays an important role for the pathogenesis of senile cataract.

R776.1