大鼠角膜碱烧伤后角膜淋巴管生成及VEGF-C和VEG-FR-3的表达

Expression of VEGF-C,VEGFR-3 and lymphangion genesis in rat cornea after alkali burn

摘要:

目的:研究大鼠角膜碱烧伤后角膜淋巴管生成及VEGF-C和VEGFR-3的表达。方法:制作角膜碱烧伤模型,应用免疫组化方法检测正常角膜及烧伤后1,3,5,7,14,21,28d角膜组织中VEGF-C和VEGFR-3的表达,免疫组化LYVE-1(淋巴管内皮特异标记物)染色观察角膜中淋巴管生成情况,电镜观察烧伤后14d角膜新生淋巴管状况。结果:角膜碱烧伤后,VEGF-C和VEGFR-3的表达明显升高,烧伤后3d,角膜中出现淋巴管。结论:角膜碱烧伤后新生淋巴管生成伴VEGF-C和VEGFR-3高表达。

Abstract:

AIM:To investigate the expression of VEGF-C,VEGFR-3 and lymphangion genesis in rat cornea after alkali burn. ·METHODS:The rat alkali burnt corneal model were made. The expression of VEGF-C,VEGFR-3 and lymphangion genesis labeled with LYVE-1 in rat cornea were detected by immunohistochemical assay at day 0 and day 1,3,5,7,14,21,28 after burn. In addition,the ultrastructure of lymphangion genesis in rat cornea was observed by electron microscope. ·RESULTS:The expression of VEGF-C and VEGFR-3 in rat cornea were increased after alkali burn. LYVE-1 were detected in rat cornea 3 days after alkali burn.·CONCLUSION:There are some new lymphangion genesis in rat cornea after alkali burn. And meanwhile,the expression of VEGF-C and VEGFR-3 in rat cornea was significantly increased.