目的:探讨重组人促红细胞生成素(recombinant human erythropoietin,rhEPO)对大鼠视网膜缺血再灌注损伤(retina ischemia reperfusion injury,RIR)中视网膜神经节细胞(retinal ganglion cell,RGC)的保护作用。方法:成年雌性SD大鼠20只,采用夹闭视网膜动脉30min造成大鼠双眼缺血再灌注模型。所有大鼠均于建模前1h给予左眼rhEPO10U(6μL),右眼给予同等剂量眼用平衡盐液。按照建模后眼球取材时间不同(1,4,7,14d)分为4组,每组5只,均于取材前4d利用荧光金(fluorogold,FG)逆行标记大鼠RGC,视网膜铺片RGC计数,比较双眼存活RGC数量。结果:rhEPO治疗眼RGC存活数多于平衡盐液对照眼。结论:rhEPO对大鼠视网膜急性缺血后RGC具有保护作用。

·AIM:To evaluate the effect of recombinant human erythropoietin(rhEPO)to the retinal ganglion cell(RGC)in retina ischemia reperfusion injury(RIR)in rats.·METHODS:The RIR model was modeled by retinal artery’s 30 minutes occlusion in 20 female SD rats.6μL/10U rhEPO was injected in left eyes and physiological saline was injected in right eyes as vehicle controls at 1 hour before modeling.20 rats were divided into four groups(1 day,4,7,14 days)by the time of drawing the optic bulb after modeling.The survival RGCs was observed.The fluorogold(FG)tracing technique was used at 4 days before drawing the optic bulb in both eyes.·RESULTS:The quantity of the RGCs in eyes treated with the rhEPO were significantly higher than those in control group.·CONCLUSION:The rhEPO has protective effect on the RGCs in RIR in rats.·

R96