目的:评价中药灯盏花提取物对高眼压状态下视网膜神经节细胞的保护作用。方法:家猫40只随机分为空白组、模型组、灯盏花素组和脑神经生长素对照组,每组10只,前房注射20g/L甲基纤维素复制急性高眼压模型,造模后1d开始前肢iv给药,1次/d,连续给药9d,观察用药前后多焦视网膜电图(mfERG)的变化。结果:造模眼压升高后mfERG一阶反应有明显变化:P1波总的反映密度减弱;同心环各环P1波反映密度均较术前减弱;第3环P1波峰潜时延长。灯盏花素iv9d后mfERG有明显的恢复:2,3环N1振幅密度与模型组及用药前比较显著增加;总环及同心环第1,2,3,4环P1波振幅密度显著增加;同心环第3环P1波峰潜时显著缩短。结论:灯盏花提取物能明显提高mfERG一阶反应N1,P1波振幅密度,缩短P1波峰潜时的作用优于脑神经生长素。

·AIM:To assess the protective effect of Erigeron breviscapus extraction on retinal ganglion cells under high intraocular pressure.·METHODS:Totally 40 cats were randomly divided into four groups,control,model,Erigeron breviscapus and cranial nerve growthine group,10 cats in each group.All of cats were injected 20g/L Methyl cellulose into anterior chamber to make cat model with acute high intraocular pressure,then,forelimb vein medicine administration was started respectively according to every experimental group from the first day after model completed,once a day,9 days continuously.At the end,the changes of multifocal electroretinogram(mfERG)were observed before and after treatment.·RESULTS:The first order reaction of mfERG had significant changes in cats with high intraocular pressure,mainly as follows:the total reaction density of wave P1 decreased and the P1 reflect density of each ring in concentric rings were less than that of preoperative,and the peak latency of the third ring prolonged.All above of mfERG showed high intraocular pressure damaged to the visual function.After treatment with Erigeron breviscapus extraction for 9 days,the mfERG was obviously improved.·CONCLUSION:It be manifested as:the wave N1 amplitude density of the second and third ring increased compared with model group and pre-medication,the wave P1 amplitude density of total ring and the first,second,third and fourth of concentric rings increased significantly,the wave P1 peak latency of the third concentric ring shorted obviously.Conclusion:Erigeron breviscapus extraction could improve wave N1 and P1 amplitude density and shorten wave P1 peak latency in the first order reaction.It illustrates that the extraction of breviscapus can protect and improve the ocular function under high intraocular pressure.·

R285.5

中国国家自然科学基金资助项目(No.30171172)~~