目的:利用激光共焦显微镜观察汉防己甲素可吸收缓释膜在活体兔眼结膜下的吸收情况。方法:按照适当比例制备汉防己甲素壳聚糖明胶缓释可吸收膜剂及空白对照缓释膜剂,植入兔眼结膜下1wk后,在激光共焦显微镜下观察缓释膜5个不同层面的吸收情况,计算吸收面积的平均百分比,并利用SPSS 18.0软件分析两组汉防己甲素缓释膜吸收的差别。结果:缓释膜干膜在激光共焦显微镜下可见到大量较均匀的细小颗粒,兔眼结膜下植入后1wk的汉防己甲素缓释膜为分布有大量吸收空泡及形态不甚规则的吸收缺损区域的半透明膜。汉防己甲素缓释膜(A)组的吸收面积平均百分比为10.5%±0.28%,无药物空白缓释膜(B)组的吸收面积平均百分比为15.0%±4.6%。经过独立样本t检验,t=1.301,P=0.000,汉防己甲素缓释膜在兔眼结膜下的吸收面积百分比较低。结论:通过激光共焦显微镜可以直观地了解缓释膜内部在吸收过程中的降解情况,且发现汉防己甲素药物对壳聚糖明胶缓释膜的生物性吸收有阻碍作用,激光共焦显微镜是一种很好的药学研究工具。

AIM:To observe the absorption of tetrandrine sustained-release absorption film in vivo in rabbit conjunctiva under the laser confocal microscopy. METHODS:Tetrandrine film prepared with an appropriate proportion of chitosan and gelatin was implanted under the conjunctiva of rabbits,blank film as control.1 week later the two groups of film was observed with the confocal microscope at five different levels.The average percentage of absorption area of the film was calculated. The difference of absorption was analyzed by the SPSS 18.0. RESULTS:Lots of uniform small particles in the dry film was observed under the laser confocal microscope.At the same time,there were some vacuoles and the absorption regions in the film under the rabbit conjunctiva.The average absorption percentage was 10.5%±0.28%in the tetrandrine film group,and 15.0%±4.6%in the control. Tetrandrine film absorption in the rabbits conjunctiva was lower,with t= 1.301 and P= 0.000. CONCLUSION:The laser confocal microscope can be used to observe the degradation of film directly,and to get the result of the tetrandrine releasing the biological absorption of the film.So it is a good tool of the pharmaceutical research.

中国2008年广东省中医药局建设中医药强省科研课题资助项目（No.2008282）