目的:通过改变四氧嘧啶给药次数探索改良制作兔糖尿病模型的方法。方法:选取健康大耳白兔36只,体质量为2～2.5kg,注射四氧嘧啶剂量均为150mg/kg,随机分为3组,每组12只,分别为一次给药组(A组),分两次给药组(B组)和分三次给药组(C组)。观测各组空腹血糖值,计算各组成模率和死亡率。结果:B组兔糖尿病模型成模率显著高于A组(P<0.01),死亡率显著低于A组(P<0.01);C组兔糖尿病模型成模率显著高于B组(P<0.01),死亡率显著低于B组(P<0.01)。结论:四氧嘧啶分次给药能提高制作兔糖尿病模型的成模率,降低死亡率并且可以保持模型的稳定。

AIM:To explore the frequency of drug injection of alloxan diabetes on the established model of rabbit. METHODS:Thirty-six healthy rabbits,weighing 2- 2.5kg,were randomly divided into one time drug injection group(group A,n = 12),two times drug injection group (group B,n = 12) and three times drug injection group (group C,n = 12).Each rabbit was injected with a total amount of 150mg/kg of alloxan.Fasting blood glucose was measured.The success rate and death rate of each group were also calculated. RESULTS:The success rate of diabetic rabbit model in group B was higher than that in group A(P<0.01) and its death rate was lower than that of group A(P<0.01);the success rate of diabetic rabbit model in group C was highest and the death rate was the lowest in three groups (P<0.01). CONCLUSION:Multiple administration of alloxan can improve success rate in establishing diabetic rabbit model with decreased death rate and increased stability.

中国西安市科技局基金资助项目(No.YF07142)