目的:分析结膜松弛症泪液中肿瘤坏死因子α(TNF-α)、白细胞介素-6(IL-6)和白细胞介素-8(IL-8)的表达量的变化。方法:收集25例结膜松弛症患者和13例正常对照组的泪液样本,毛细管从每眼收集15μL泪液,采用放射免疫测定法测定结膜松弛症组和正常对照组的泪液中TNF-α,IL-6和IL-8的浓度并进行统计学分析。结果:结膜松弛症患者TNF-α浓度为19.05±6.35ng/mL,正常对照组TNF-α浓度为14.13±7.76ng/mL;IL-6浓度为13.16±8.97pg/mL,正常对照组IL-6浓度为4.24±3.97pg/mL;IL-8浓度为34.40±20.73pg/mL,正常对照组IL-8浓度为18.31±11.62pg/mL;两组之间比较差异均有统计学意义(P<0.05)。结论:应用放射免疫的方法可检测泪液中的细胞因子含量,结膜松弛症组泪液中三种细胞因子的含量显著增高提示结膜松弛症可能与细胞因子的增加及免疫反应有关。

AIM:To analyze the expression changes of tumor necrosis factor α (TNF-α),interleukin-6 (IL-6) and interleukin-8 (IL-8) in conjunctivochalasis tears. ·METHODS:Tears samples were obtained from 25 conjunctivochalasis cases and 13 normal controls. Fifteen microliters of tears were collected by microcapillary tubes from each eye. The tear concentrations of TNF-α,IL-6 and IL-8 were analyzed by radioimmunoassay method and compared the statistical difference between conjunctivochalasis and normal controls. ·RESULTS:The concentration of TNF-α in conjunctiv-ochalasispatients was 19.05±6.35ng/mL,in normal control group was 14.13±7.76ng/mL; conjunctivochalasis concentration of IL-6 was 13.16±8.97pg/mL,normal control group concentration of IL-6 was 4.24±3.97pg/mL; conjunctivochalasis concentration of IL-8 was 34.40±20.73pg/mL,normal control group concentration of IL-8 was 18.31±11.62pg/mL. The difference between the two groups was significant (P<0.05). ·CONCLUSION:Radioimmunoassay method could detect levels of cytokines in tears. The tear concentrations of the three cytokines from the conjunctivochalasis group were significantly higher,which may indicate conjunctivochalasis is related to the increase of cytokines and immune response.

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中国上海市卫生局中医药科研基金资助项目(No.2008Q006)~~