目的:探讨谷氨酰胺(Gln)体外对晶状体上皮细胞HSP70和NF-κB的作用。方法:采用ELISA法检测在MEM培养液里培养3,12和24h晶状体上皮细胞HSP70和NF-κB的表达水平。结果:谷氨酰胺注射各组HSP70蛋白的表达量均显著高于对照组(P<0.05),谷氨酰胺注射各组NF-κB的表达较对照组显著降低(P<0.05)。结论:谷氨酰胺能诱导晶状体上皮HSP70蛋白的表达,抑制NF-κB的表达。

AIM:To study the effect of glutamine(Gln) on the expression of heat shock protein 70(HSP70) and NF-κB in vitro. ·METHODS:HSP70 and NF-κB expression was detected by ELISA in different times(3,12,24 hours)after the lenses of rats were cultivated in MEM cultural medium. ·RESULTS:The expression of HSP70 in the Gln group was significantly higher than that of the control group(P<0.05;On the contrary,the expression of NF-κB in the Gln group was significantly lower than that of control group(P<0.05). ·CONCLUSION:Gln can induce the expression of HSP70 in the lens epithelial cells of rat and inhibit the expression of NF-κB.

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