目的:探讨结膜印迹细胞学检查在干眼症诊断中的作用。方法:收集2008-04/2008-09眼科门诊干眼症患者36例72眼,正常人10例20眼,采用结膜印迹细胞学检查联合PAS染色,观察各组结膜上皮细胞和杯状细胞形态及数量变化情况。结果:在形态学方面,97%的正常人表现为Nelson分级中的0级,84%轻度干眼症患者表现为Nelson分级中的1级,79%中度干眼症患者符合Nelson分级中的2级,98%重度干眼症患者符合Nelson分级中的3级;在高倍镜下任意选取五个视野,统计杯状细胞的数量,轻度干眼症组(101.67±15.64),中度干眼症组(40.77±5.39)及重度干眼症组(6.36±3.61)较正常组(196.10±22.53)相比,杯状细胞数量明显减少,P<0.01,重度与轻中度干眼症组比较,前者杯状细胞数量明显少于后两者,差异有统计学意义(P<0.01)。结论:结膜印迹细胞学检查操作简单易行,结果客观精确,联合其他检查方法用于干眼症诊断,有着良好的应用前景。

·AIM:To evaluate the effect of conjunctival blotting cytological examination in dry eye diagnosis.·METHODS:Totally 36 patients 72 eyes with dry eye syndrome from April 2008 to September 2008 and 10 normal volunteers 20 eyes were analyzed.Conjunctival blotting cytology combined PAS staining were applied.Conjunctival epithelial cells and the number,morphology and changes of goblet cells were observed.·RESULTS:Morphologically,97% of the normal volunteer presented the Nelson grade 0,84% of mild dry eye patients presented the Nelson grade 1,79% of moderate dry eye patients presented the Nelson grade 2,98% of severe dry eye patients presented the Nelson grade 3;The number of goblet cells was calculated using five visual fields randomly under high magnification.There was significantas decrease in dry eye group(mild:101.67±15.64,moderate:40.77±5.39,severe:6.36±3.61)compared with normal group(196.10±22.53,P<0.01);There was significant decreased in severe dry eye group than in mild or moderate dry eye group and the difference was statistically significant(P<0.01).·CONCLUSION:Conjunctival blotting cytological examination is asimple,accurate and objective method,when combined with other examination methods,it has good prospects for diagnosis of dry eye.·

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