目的:定量测定结缔组织生长因子(connective tissue growth factor,CTGF)和基质细胞衍生因子1(stromal cell-derived factor-1,SDF-1)在糖尿病视网膜病变(diabetic retinopathy,DR)患者玻璃体中的质量浓度,探讨其在糖尿病(diabetic retinopathy,DR)发病机制中的作用。方法:采用双抗体夹心酶联免疫吸附测定法(enzyme linked immunosorbent assay,ELISA)定量检测33例增生型糖尿病视网膜病变(proliferative diabetic retinopathy,PDR)、5例单纯型糖尿病性视网膜病变组(background diabetic retinopathy,BDR组)及5例正常对照组玻璃体中CTGF的质量浓度。结果:PDR组玻璃体中CTGF质量浓度大于对照组(P<0.01)、BDR组(P<0.01)。PDR组玻璃体中SDF-1质量浓度大于BDR组(P<0.05)。结论:SDF-1,CTGF在DR发展过程中起着一定的作用。

AIM:To measure the concentration of connective tissue growth factor(CTGF) and stromal cell-derived factor-1(SDF-1) in vitreous and to observe its relationship with diabetic retinopathy(DR) formation.METHODS:By enzyme linked immunosorbent assay(ELISA) method,the concentration of SDF-1 and CTGF in vitreous in 33 cases of proliferative diabetic retinopathy (PDR),5 cases of background diabetic retinopathy(BDR)and 5 cases of normal control were measured.RESULTS:CTGF concentration in vitreous of PDR patients was significantly increased compared with that in normal group(P<0.01),BDR patients(P<0.01).SDF-1 level in vitreous of PDR compared with BDR was significantly increased (P<0.05).CONCLUSION:SDF-1 and CTGF may play certain role in the development of PDR.