目的:研究Lat-B对家兔眼内压、葡萄膜巩膜通路的影响。方法:兔眼局部应用Lat-B,BSS和DMSO,于用药前1h及用药后1,2,3,4,5,6,24h测量眼压后部分免疫荧光组通过前房内注入微量示踪剂异硫氰酸荧光素牛血清白蛋白(fluorescein isothiocyanate bovine serum albumin,FITC-BSA),于点眼后2,4,6,8,10h各处死家兔2只,摘除双侧眼球作冰冻切片,于荧光显微镜下观察并确定睫状体、脉络膜上腔、前、后巩膜和脉络膜的荧光强度等级。结果:Lat-B滴眼后处理眼眼压不同时间点与滴眼前比较眼压呈下降趋势,差异有显著性(P<0.05),1h即可引起眼压降低,24h仍有效,BSS组与DMSO组未引起眼压下降;葡萄膜巩膜途径通路3组之间在睫状体、脉络膜上腔、前、后巩膜和脉络膜的荧光强度,各部位均为Lat-B组最强,Lat-B与BSS组和DMSO组相比差异均有显著性(P<0.05),BSS组和DMSO组相比差异无显著性。结论:Lat-B能够有效降低眼压,1h即可起效,且24h仍有效。Lat-B可以增加葡萄膜巩膜途径通路的房水流出率,具有治疗青光眼的应用前景,尤其是正常眼压性青光眼、开角性青光眼。

AIM:To determine the effects of Latrunculin-B (Lat-B )on intraocular pressure (IOP),aqueous humor flow (AHF),uveoscleral outflow.METHODS:Topical Lat-B was administered to one eye,and vehicle to the other.IOP was measured by Goldmann tonometry.The IOP were measured at 1 hour before administration and 1 hour,2,3,4,5,6,24 hours after administration respectively;immunofluorescence was detected by instillation of FITC-BSA into anterior chamber,at 2,4,6,8,10 hours,2 rabbits were killed and frozen section of the eyes were done,and then the fluorescence intensity of ciliary body,epichoroidal space,anterior and posterior sclera were detected respectively.RESULTS:Lat-B could decrease the IOP,compared with the IOP before drug administration,the difference was significant(P<0.05),and this effect occurred within 1 hour,some IOP effect may remained for 24 hours,but the IOP of BSS group and DMSO group were not decreased.The fluorescence intensity of ciliary body,epichoroidal space,anterior and posterior sclera after drug administration of Lat-B were higher than that of BSS group and DMSO group(P<0.05),there was no difference between BSS group and DMSO group(P>0.05).CONCLUSION:Lat-B can decrease IOP and this effect occurred within 1 hour,remained effective at 24 hours;Lat-B may be used in the therapy of glaucoma by increasing the uveoscleral outflow facility,especially in NTG and POAG.

R775

中国湖南省卫生厅资助课题(No.B2005105)~~