目的:探讨HIF-1α和caspase-9表达与高眼压视网膜损伤的关系。方法:大鼠60只随机分为6组,每组10只20眼。分别为假手术对照组;高眼压3,7,14,21,28d组。巩膜静脉烧烙法制作高眼压模型。应用免疫组织化学法,RT-PCR法,Western印迹法检测各组视网膜组织中HIF-1α和caspase-9基因mRNA及蛋白表达。结果:HIF-1α和casepase-9阳性染色主要位于视网膜内层即视网膜节细胞层和内颗粒层。HIF-1α和caspase-9mRNA和蛋白在正常视网膜中有低浓度的表达,高眼压3d后,表达明显上升,HIF-1α到7d时达到高峰,caspase-9到14d达到高峰,随后有所下降,但仍明显高于正常组的表达水平,差异有统计学意义。结论:HIF-1α和caspase-9是青光眼神经节细胞凋亡的发生和进展中重要的病理生理机制。

AIM:To research the mRNA and protein expressions of hypoxia-inducible factor-1α(HIF-1α) and caspase-9 in normal retina and ocular hypertention retina,investigate the relation of glaucomatous retinal degeneration and HIF-1α and caspase-9 signal transduction path.METHODS:Coagulating two episcleral veins unilaterally in rats with electric coagulator to establish the glaucoma model.Use immunochemistry,PT-PCR and Western Blot analysis to examine the expression of HIF-1α and caspase-9 genes.RESULTS:The expression of HIF-1α and caspase-9 were very faint immunostaining in normal retina.In the glaucomatous retina,immunostaining for HIF-1α and caspase-9 was predominant in the retinal ganglion cell layer and the inner nuclear.In RT-PCR and Western Blot,HIF-1α and caspase-9 genes mRNA and protein were faintly expressed in normal rat retina,and the expression of them increased in IOP elevation model rats with the time going on.The peak expression of HIF-1α was on the 7th day after IOP elevated.The peak expression of caspase-9 was on the 14th day then decreased but still maintained at a higher level.The expression among experimental groups was statisticlly different compared with the control group.CONCLUSION:HIF-1α and caspase-9 participates in signal transduction of retinal neurons apoptosis during retinal injury on chronic high ocular pressure.

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