目的:探讨汉防己甲素(tetrandrine,Tet)联合5-FU聚乳酸微球对兔增生性玻璃体视网膜病变(proliferati vevitreoretinopathy,PVR)IL-1β和TNF-α表达的影响。方法:随机分为A,B,C3组,建立兔眼外伤性PVR模型,向玻璃体中后部注入药物,A组注入含有Tet联合5-氟尿嘧啶(5-FU)聚乳酸微球的BSS悬浮液0.2mL,B组注入含有5-FU聚乳酸微球的BSS悬浮液0.2mL,C组注入25mg无药物聚乳酸微球的BSS悬浮液0.2mL,术后每天观察眼底变化,必要时行B超检查直到注药后第28d。分别于术后7,14,28d等3个时间点抽取各术眼玻璃体液0.2mL,酶联免疫吸附法(ELISA)检测玻璃体液中TNF-α,IL-2的含量。结果:Tet联合5-FU聚乳酸微球组与5-FU聚乳酸微球组及空白微球组相比,其玻璃体液中TNF-α,IL-2等炎性因子的含量也显著低于其他两组,方差分析P<0.01,差异有统计学意义。结论:Tet在眼内能够降低炎性因子TNF-α和IL-1β的表达,说明Tet可能通过在PVR炎症期发挥抗炎作用,抑制眼内炎症因子的释放,减弱炎症因子对炎性细胞的激活和趋化,从而起到降低PVR发生率的协同作用。

AIM:To investigate the change of the IL-1β and TNF-α expression by tetrandrine (Tet) combined 5-fluorouracil(5-FU) polylactic acid microspheres on the rabbit proliferative vitreoretinopathy(PVR).METHODS:Ocular trauma PVR rabbit model was constructed.These rabbits were divided randomly into three groups:A group was injected Tet and 5-FU BSS polylactic acid microspheres (0.2mL)into posterior vitreous;B group was injected 5-FU BSS polylactic acid microspheres 0.2mL into posterior vitreous,C group was injected drug-free polylactic acid 25mg microspheres BSS 0.2mL into posterior vitreous.After injection,fundus observation was performed every day up to 28 days,and B ultrasonic inspection was used.At postoperative day 7,14 and 28,vitreous humor (0.2mL)was extracted from posterior vitreous,and enzyme-linked immunosorbent assay (ELISA) detection of TNF-α and IL-2 was used.RESULTS:Compared Tet combined 5-FU polylactic acid microspheres group with 5-FU group of polylactic acid microspheres or microspheres group,TNF-α and IL-2 content in vitreous humor in Tet combined 5-FU polylactic acid microspheres group was significantly lower than that in the other two groups(P<0.01).There were significantly statistical differences.CONCLUSION:Tet can reduce inflammatory mediators TNF-α and IL-1β expression,which indicates that Tet may play an anti-inflammatory role in the PVR.Tet has many advantages such as inhibiting the release of inflammatory factors,decreasing inflammatory cytokines activation and chemotaxis on the inflammatory cells.All this results in the decrease of the recurrence rate of PVR.

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中国山东省自然基金资助项目(No.Y2006C100)~~