AIM:To explore the effect of mitomycin C(MMC) in glaucoma filtration surgery.METHODS: The effect of MMC in the glaucoma filtration surgeries in 35 eyes of 32 patients was observed.RESULTS: In the average two years’ follow-up, visual acuity was improved in 21 eyes, remained unchanged in 14 eyes, type Ⅰfiltration bleb occurred in 18 eyes, type Ⅱ in 15 eyes and type Ⅲ in 1 eye. One eye had normal intraocular pressure without filtration bleb.CONCLUSION: The application of MMC in glaucoma filtration surgery is an effective method for glaucoma.