血管抑素对角膜新生血管和VEGF表达的作用

Effect of angiostatin on rat corneal neovas-cularizasion and the expression of vascular endothelial growth factor

摘要:

目的:探讨血管抑素(angiostatin,AS)对鼠碱烧伤角膜新生血管(corneal neovascularization,CNV)及血管内皮细胞生长因子(vascular endothelial growth factor,VEGF)表达的作用,探讨 AS对CNV的作用及机制。方法:SD大鼠105只随机分为正常对照组(5只)、生理盐水组、地塞米松组、AS1组、AS2组(各25只),除正常组外,实验各组制作左眼碱烧伤CNV模型,分别予生理盐水、1g/L地塞米松液、10μg/mLAS液、20μg/mLAS液点眼,4次/d,于碱烧伤后1,3,7,14,21d运用裂隙灯、免疫组织化学方法观察新生血管长度及面积、VEGF的表达。结果:AS治疗组碱烧伤后第3d起各时间点CNV面积均明显小于盐水组(P<0.05)。碱烧伤后各时间点AS治疗组VEGF表达量均明显少于盐水组(P<0.05)。结论:AS能显著抑制碱烧伤CNV,可能与其抑制VEGF的表达有关。

Abstract:

AIM:To investigate the anti-angiogenesis effect of angiostatin(AS) on rat corneal neovascularization(CNV) and the expression of vascular endothelial growth factor(VEGF).·METHODS: CNV models were established in 105 SD rats,which were randomly divided into normal group(5 SD rats) and experimental group(100 SD rats,induced by alkali burn):normal saline group, 1g/L dexamethasone group, and different concentration angiostatin-treated group(group AS1 10μg/mL and group AS2 20μg/mL). Slit-lamp microscope was performed and the area of CNV was calculated on 3, 7, 14, 21 day after injury. Then 5 rats were randomly sacrificed and the corneas were taken for histopathological and immunohistochemistry examina-trion.·RESULTS: The area of CNV in the AS groups was smaller than that in the normal saline group 3 days after cautery(P<0.05). The expression of VEGF had statisti-cally significant difference between AS group and normal saline group(P<0.05).·CONCLUSION: AS can effectively inhibit rat CNV after alkali burn. The mechanism probably is down-regulation of the expression of VEGF.