AQP-1和VEGF在大鼠视网膜缺血再灌注损伤中的表达

Expression levels of AQP-1 and VEGF in rat retinal ischemia reperfusion injury

摘要:

目的:研究大鼠视网膜缺血再灌注损伤后水通道蛋白-1和血管内皮生长因子的表达情况并探讨二者在视网膜缺血再灌注损伤中表达的意义。方法:通过提高眼内压的方法建立大鼠视网膜缺血再灌注模型,于术后6,12,24,48h;3,7d用免疫组化的方法检测水通道蛋白-1和血管内皮生长因子在大鼠视网膜的表达情况,并以正常大鼠视网膜作为对照。结果:正常对照组大鼠视网膜未能检测到血管内皮生长因子的阳性表达,缺血再灌注12h后开始出现表达,48h达高峰,且差异具有统计学意义(P<0.01)。水通道蛋白-1在正常对照组可见到阳性表达,缺血再灌注组随时间增长表达不断增强,7d在外层视网膜也出现表达,差异有统计学意义(P<0.01)。结论:水通道蛋白-1和血管内皮生长因子在视网膜缺血性疾病中起着重要的作用,二者可能共同影响了视网膜的水代谢过程。

Abstract:

AIM:To investigate the expression levels and signifi-cance of aquaporin-1 (AQP-1) and vascular endothelial growth factor(VEGF) in the retinal ischemia reper-fusion injury(RIRI). METHODS:The model of transient RIRI of the rat retina was constructed by elevation of the intraocular pressure .The expression levels of AQP-1 and VEGF was measured 6, 12, 24 and 48 hours, 3, 7 days after retinal ischemia using immunohistochemical staining. Normal retina was treated as control group. RESULTS: VEGF positive cells were not found in normal group and began to express after 12 hours in ischemia group, increased gradually and reached maximal levels 48 hours after RIRI. There was statistical difference between 48 hours group and other ischemia reperfusion group (P<0.01). AQP-1 positive cells were found in the outer retina in normal group, increased gradually in ischemia reperfusion group and also were found in the inner retina 7 days after RIRI, and the difference was significant (P<0.01). CONCLUSION: AQP-1 and VEGF may play an important role in various blinding ischemic ocular diseases, and co-effect the water metabolism of retina.