目的:探讨尾加压素Ⅱ(urotensinⅡ,U-Ⅱ)对晶状体上皮细胞(lens epithelial cell,LEC)增殖的影响。方法:采用不同浓度的U-Ⅱ与体外培养的LEC共同孵育后,用MTT法检测LEC的活性,并用3H-胸腺嘧啶(3H-TdR)掺入法检测LEC增殖。结果:10-9mol/L U-Ⅱ组、10-8mol/L U-Ⅱ组、10-7mol/L U-Ⅱ组和10-6mol/L U-Ⅱ组的吸光度值均显著高于对照组(P<0.05,P<0.05,P<0.01,P<0.01)。10-10mol/L U-Ⅱ组、10-9mol/L U-Ⅱ组、10-8mol/L U-Ⅱ组、10-7mol/L U-Ⅱ组和10-6mol/L U-Ⅱ组的3H掺入放射活性均显著高于对照组(P<0.01),分别是对照组的1.36倍、1.40倍、1.45倍、1.59倍和1.66倍。呈浓度依赖关系。结论:U-Ⅱ具有促进LEC增殖的作用。

·AIM:To study the effect of urotensin Ⅱ(U-Ⅱ),a new biological activity substance,on proliferation of lens epithelial cell(LEC).·METHODS:Different concentrations of U-Ⅱ were incubated with in vitro cultured LEC in vitro.The activities of LEC were detected via methyl thiazolyl tetrazolium(MTT).The proliferations of LEC were detected via 3H-TdR.·RESULTS:The absorbences of groups 10-9mol/L U-Ⅱ,10-8mol/L U-Ⅱ,10-7mol/L U-Ⅱ and 10-6mol/L U-Ⅱ were higher than that in control group(P<0.05,P<0.05,P<0.01,P<0.01);the radio-activities of 3H-TdR in groups 10-10mol/L,10-9mol/L U-Ⅱ,10-8mol/L U-Ⅱ,10-7mol/L U-Ⅱ and 10-6mol/L U-Ⅱ were higher than that in control group(P<0.01),which were 1.36,1.40,1.45,1.59 and 1.66 times respectively,with a dose-dependent manner.·CONCLUSION:U-Ⅱ can induce proliferation of LEC.·

R776.1

中国福建省中医药重点资助项目(No.Wzzb0601)~~