目的:环孢素A(Cyclosporin A,CsA)滴眼液在小鼠角膜上皮愈合过程中上调水通道蛋白3(aquaporin protein3,AQP3)表达的作用机制。方法:采用机械法刮除小鼠角膜上皮的方法建立模型,观察角膜上皮修复情况。取角膜行AQP3免疫组化染色,观察AQP3表达。结果:角膜上皮基底细胞层可见AQP3的表达。应用125,250,500,1000mg/LCsA滴眼液点眼后AQP3的表达呈递增趋势。角膜上皮损伤后用500mg/LCsA滴眼液点眼,损伤后6h实验组与对照组愈合面积比较无统计学差异,而损伤后12,18,24h比较均有显著统计学差异(P<0.05)。结论:应用500mg/LCsA滴眼液点眼,可通过增加AQP3表达而加快小鼠角膜上皮损伤的修复。

·AIM:To learn the up-regulation of aquaporin protein 3(AQP3)expression in the corneal epithelial healing process of mice by cyclosporin A(CsA)eye drops.·METHODS:Models were established in the mechanical curettage method of corneal epithelium in mice to observe the repair of corneal epithelium.Corneas were treated with AQP3 immunohistochemical staining to observe the expression of AQP3.·RESULTS:AQP3 expression could be seen in the basal cell layer of the cornea.After applications of 125,250,500,1000mg/L CsA eye drops,the AQP3 expression was increased progressively.When corneal epithelium injury was treated with 500mg/L CsA eye drops,there was no statistical difference in healing size between the experimental group and control group 6 hours after injury,while 12,18,24 hours after injury there was a statistically significant difference(P<0.05).·CONCLUSION:500mg/L CsA eye drops can accelerate mice corneal epithelial damage repair by increasing the expression of AQP3.·

R779.1