目的:观察蛹虫草提取物(cordyceps militaris extract,CME)联合地塞米松(dexamethasone,Dex)对大鼠角膜新生血管的抑制作用。方法:对48只大鼠采用碱烧伤法制作大鼠角膜新生血管模型,后随机分为3组:A组为模型对照组,B组为CME治疗组,C组为CME联合Dex治疗组。造模后每组均隔日注射给药,A组给予生理盐水结膜下注射,B组给予CME10mg球结膜下注射,C组给予CME10mg球结膜下注射,另外给予0.25g/L Dex滴眼液点眼,3次/d。于术后4,8,14d测量角膜新生血管的生长面积,并测量角膜组织中VEGF及其mRNA的表达情况。结果:造模后联合用药组的大鼠角膜新生血管生长面积较模型对照组及CME治疗组明显减少,差异均具有统计学意义(P<0.05)。结论:CME联合Dex更能明显抑制碱烧伤所致大鼠角膜新生血管的生长。

·AIM:To observe the inhibition effect on rat corneal neovascularizition(CNV)by drug combination of the cordyceps militaris extract(CME)and dexamethasone(Dex).·METHODS:CNV model was induced by alkaline burn of the cornea with 1mol/L NaOH.Forty-eight rats were divided into three groups randomly after modeling,A was model group,B was CME treatment group,and C was combination treatment group.All groups received medication by subconjunctival injection every other day after alkaline burn,group A received saline,group B received 10mg CME,group C also received 10mg CME,and received 0.25g/L Dex eyedrops three times each day,each injection volume was set at 0.1mL.On the 4,8,14 days,CNV was observed,and the expression of vascular endothelial growth factor(VEGF)and it’s mRNA in the cornea were detected.·RESULTS:After modeling,rat CNV of the combination group was significantly reduced than the model control group and CME treatment group,the differences were statistically significant(P<0.05).·CONCLUSION:CME combining with Dex can inhibit alkaline burn-induced CNV growth more effectively.·

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