目的:研究慢性高眼压大鼠视网膜HSP70表达的变化及替普瑞酮的影响。方法:Wistar大鼠70只随机分为3组:空白对照组10只;慢性高眼压模型组30只;慢性高眼压模型+GGA组30只。采用烧灼巩膜浅层静脉的方法制备大鼠慢性高眼压模型。模型成功后给予替普瑞酮800mg/(kg.d)每日灌胃。应用免疫组织化学方法观察应用及未应用替普瑞酮的慢性高眼压大鼠视网膜不同时点的差异及HSP70表达的变化。结果:平均眼压高于正常眼压40%的手术眼为造模成功。与正常对照组相比,慢性高眼压大鼠应用与未应用替普瑞酮者,视网膜均随着高眼压时间的延长逐渐出现形态学变化,于高眼压的21d和28d视网膜变薄,节细胞数量减少;在此过程中,HSP70表达增多。慢性高眼压大鼠视网膜应用替普瑞酮者与未应用提普瑞酮者相比,其形态学变化较小,而HSP70表达则明显增多,两者差异具有统计学意义(P<0.01)。结论:替普瑞酮通过上调HSP70表达发挥对慢性高眼压视网膜的保护作用。

·AIM:To study the effects of geranylgeranylacetone(GGA)on the expression of heat shock protein70(HSP70)on retinal ganglion cells(RGC)in rats with chronic intraocular pressure(IOP)elevation.·METHODS:Seventy Wistars were divided into blank control group(10 rats),chronic hypertension group(30 rats)and GGA group(30 rats).Chronic hypertension was created by cauterizing the superficial scleral veins.800mg/(kg·d)GGA was given by oral daily after cauterization.Immunohistochemistry was used respectively to observe the changes of expression of HSP70 in the model rats and GGA interference rats at different time points during the course of chronic IOP elevation.·RESULTS:The successful model was identified as the IOP over 40% of normal rats.The retinal thickness was significantly reduced in model group and model+ GGA group compared with normal rats from 21 days through 28 days after cauterization(P<0.05),and that of model rats was obviously decreased in comparison with model+ GGA rats(P<0.05).The number of ganglion cells was significantly decreased in model rats and model+ GGA rats compared with normal rats from 21 days and 28 days.The stronger expression intensity(IOD)value was seen for HSP70 in the model+ GGA rats by immunochemistry(P<0.01).·CONCLUSION:Systemic administration of GGA protects retina from chronic IOP elevation by regulating the expression of HSP70.·

中国辽宁省科技计划资助项目（No.2009225021）